Tangential Flow Filtration for efficient depletion of nanoparticles contained in Fetal Bovine Serum
Sirin Korulu Koc, Jevgenia Dõrina, Paolo Guazzi; HansabioMed Life Sciences
Fetal Bovine Serum (FBS) or Fetal Calf Serum (FCS) are commonly used supplements in cell culture media.
However, using FBS, nanoparticles of bovine origin, such as lipoproteins and EVs, are introduced into the
culture medium, where they not only can modulate the signaling of the recipient cells but also contaminate
the cell-derived vesicle preparation, co-isolating with them during the EV purification phase.
To date, several supplements or EV depleted sera are commercially available, as well as several protocols
have been developed to remove FBS nanoparticles, mainly based on ultrafiltration or ultracentrifugation
In this application note, we show how our TFF-EVs can be used to remove nanoparticles from FBS in one single
filtration shot, working directly under the safety cabinet. A simple, fast and inexpensive way to drastically
reduce the presence of nanoparticles in FBS, without impacting the cell growth and viability, and by preserving